Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Comp Neurol. 2014 Jul 1;522(10):Spc1. doi: 10.1002/cne.23588

Figure 2.

Figure 2

PACAP is found in melanopsin immunoreactive RGC’s of the macaque monkey. A. Melanopsin immunoreactivity(magenta) (visualized using the C-terminal directed anti-melanopsin antibody) shown in a flat-mount preparation of the Macaca mulatta (MM1) retina. The image represents a montage of 4 images each representing a Z-stack of 35 sections covering the depth required to ensure that both inner and outer stratifying melanopsin processes are visible. The melanopsin cells are numbered 1-9. Note the weak staining of two inner stratifying cells (2 and 9). B. PACAP(green) staining of the same piece of retina as shown in A with cells numbered as in A (1-9). Note the low level of PACAP found in cell 9.C.Extended view representing the same Z-stack of a total of 35 images of the cells shown in A-B presented in an XY (C), XZ (C1) and ZY (C2) plane to illustrate dendritic stratification. Melanopsin (magenta) and PACAP (green) co-stored in the same RGCs numbered 1-9. Scale bars: 80 μm. GCL; ganglion cell layer, INL; inner nuclear cell layer.