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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Comp Neurol. 2014 Jul 1;522(10):Spc1. doi: 10.1002/cne.23588

Figure 6.

Figure 6

PACAP containing retinal projections innervate the lateral geniculate nucleus(LGN) of the macaque monkey. A-B. Retinal projections visualized by CtB and PACAP immunostaining of the mid ipsilateral (A) and contralateral (B) LGN innervating layers II, III and V on the ipsilateral side and layers I, IV, and VI on the contralateral side (the images in A and B consist of 6×8 tiled images which were stitched together). C-H. High magnification images of the six sublayers on the ipsilateral side taken within the area indicated by the large box in A. Co-localization between PACAP and CtB was determined using the co-localization plug-in in Fiji software (see Materials and Methods) and is shown in white (exemplified by arrows in 6C-P). I.PACAP and CtB colocalization in the ventral lateral area of layer VI indicated by the small box in A shown at a higher magnification. J-O. Images of the six sublayers on the contralateral side taken within the area indicated by the large box in B.P.A higher magnification image from the ventral lateral area of layer VI indicated by the small box in B. Note that autofluorescent cell bodies (exemplified by arrowheads in 6C-P)found throughout the LGN appear as co-localization since they are seen with both filter settings. Scale bars: A-B,200 μm; C-H and J-O,50 μm; I and P, 25μm.