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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Comp Neurol. 2014 Jul 1;522(10):Spc1. doi: 10.1002/cne.23588

Figure 8.

Figure 8

PACAP containing retinal projections representing the melanopsin ipRGCs innervating the olivary pretectal nucleus (PON).A. Retinal projections visualized by CtB immunostaining in the PON. The image consists of 3×3 tiled confocal images stitched together for better overview. B. Same section as in A co-stained for PACAP showing that PACAP was found in both retinal projections and in cell bodies located within the PON as seen at higher magnification (C-E) and ultrahigh (F-H) magnification representing the area indicated by the square in E. Co-localization between CtB and PACAP containing retinal projections is shown in white (determined using the colocalization module in ImageJ, see Material and Methods). Scale bars: A-B; 100 μm, C-E; 50 μm, F-H; 10 μm.