Table 1.
Table of primary antibodies used
| Antigen | Immunogen | Manufacture | Dilution used |
|---|---|---|---|
| PACAP Code MabJHH1 |
PACAP1-38 | In-house | 1:5 |
| Melanopsin C- terminal Code no: 5J68 |
C-terminal part of human melanopsin (amino acid sequence of the fusion protein ; see methods) |
In-house | 1:10000 |
| Melanopsin N- terminal Code no: hNA |
peptide consisting of 19 amino acid residues, MNPPSGPRVPPSPTQEPSC |
In house by Dr. King-Wai Yau, John Hopkins University |
1:500 |
| anti-CtB antibody (code no. 703, Lot #7032E |
aB subunit pentamer of cholera toxin (choleragenoid) |
List Biologicals, Champbell, CA, USA, |
1:1000 |