Figure 3.

Accumulation of H₂O₂ in Campylobacter jejuni cultures. (A) Quantification of the concentrations of H₂O₂ that accumulated in the C. jejuni strains after exposure to the oxidant. (B) Accumulation of H₂O₂ in the cultures of the ΔmfrA strain after the addition of iron in two doses (MH + Fe and MH + Fe [2×], which correspond to 40 μmol/L and 80 μmol/L iron, respectively) and desferal, respectively. A similar analysis could not be performed on the wildtype, because H₂O₂ levels were below the detection limit. Dashed line indicates the detection limit of the assay. Statistically significant (P < 0.05) differences are highlighted by “*”. Assays were repeated three times independently and samples were tested in three replicates per experiment. Data are presented as mean ± standard deviation.