Table 1.
Bacterial strains, plasmids, and primers used in this study. Restriction enzymes and restriction sites in the primer sequences are underlined.
| Strain, plasmid, or primer | Properties/Sequence | Source or reference |
|---|---|---|
| Strains | ||
| Campylobacter jejuni NCTC-11168 | Wild-type strain | Dr. Q. Zhang |
| ΔmfrA | Chloramphenicol resistance gene inserted in the mfrA (catr) | Weingarten et al. (2009) |
| C-ΔmfrA | Complementation strain. Kanamycin resistant (Kanr) and catr | This study |
| Escherichia coli DH5α | Library efficiency competent cells for cloning | Invitrogen |
| Plasmids | ||
| pIK01 | pRY108+mfrA used for constructing C-ΔmfrA | This study |
| pRY108 | Vector used in complementation studies | Dr. Q. Zhang |
| Primers | ||
| C-mfrA-f | ACGAGGATCCAATGCAATTTATGAATGGAG (BamHI) | This study |
| C-mfrA-r | GTCAGGTACCACAAAT TGCAGATTGACAAG (KpnI) | |
| mfrA-RT-f | GGGCATTTAGCAGGCATTG | Guccione et al. (2010) |
| mfrA-RT-r | GACCATTTCCGCCATTATTTG | |
| katA-RT-f | CAGTAGCAGGTGAAGCAGGTG | IDT1 |
| katA-RT-r | GCGGATGAAGAATGTCGGAGTG | |
| perR-RT-f | GCTACTCCGCAAAGACTATGTG | IDT1 |
| perR-RT-r | AGACAGATGATTGACGAGATTG | |
| fur-RT-f | CCATTTCTTTTGGTTCAGCA | IDT1 |
| fur-RT-r | GCAATCAAGGCTTGCTGTCT | |
| rpoA-RT-f | ATTACAACATCTGCTTATACG | IDT1 |
| rpoA-RT-r | TCTACTATTTCTTTATTTGATTCG | |
1
Integrated DNA Technologies, Inc.