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. 2014 Feb 7;3(2):182–195. doi: 10.1002/mbo3.162

Figure 3.

Figure 3

Introduction of a complete sfa locus from Staphylococcus aureus into Staphylococcus lugdunensis leads to SA production and growth in iron-restricted media. (A) Chrome azurol S (CAS) assay demonstrates that culture supernatants of S. lugdunensis lack siderophore activity, whereas introduction of the plasmid carrying the S. aureus sfaA-D genes into S. lugdunensis allows for the production and secretion of siderophore into culture supernatants. (B) Growth of S. lugdunensisWT strain containing either vehicle (pLI50) or plasmid carrying the staphyloferrin A biosynthetic genes (sfaA-D) in C-TMS plus 20% serum. (C) Agar plate bioassays demonstrating that the culture supernatant of S. lugdunensis does not promote the growth of iron-restricted S. aureus strains, while the supernatant of S. lugdunensis carrying the sfa gene cluster, which generates SA, can promote the growth of iron-restricted S. aureus in an hts (encodes the SA transporter)-dependent manner. Ferric citrate was used as a positive control in the experiment. All data points represent average values for at least three independent biological replicates, and error bars represent standard deviation from the mean.