Figure 4.

des expression and autoradiogram of the products of [¹⁴C]palmitate labeling of a cerulenin-resistant strain. (A) Cells of B. subtillisBLUP87 (fabF1 cer^r, amyE::Pdes-lacZ) were grown in MM at 37°C to an OD₅₂₅ of 0.35 and then were treated with cerulenin 2.5 μg mL⁻¹ (white circles) or untreated (black circles). Cultures were further transferred to 25°C. β-galactosidase specific activities (in Miller units, MU) were determined at the indicated time intervals. Dotted lines: OD_525, solid lines: β-galactosidase specific activities. Values are representative of three independent experiments. (B) UFAs synthesized by strain BLUP87 after a temperature downshift, in the presence or the absence of cerulenin. Cultures were challenged with [¹⁴C]palmitate as described in Figure 2B. About 11,000 cpm of radioactivity was loaded into all lanes. (C) Percentages of UFA synthesis. The radioactivity levels of the spots of the UFA and SFA shown in panel B were quantified by ImageQuant 5.2. Results are expressed as percentages of the total methyl esters recovered. Values are the average of three independent experiments.