Figure 5.

Effect of plsC depletion on des transcription. BLUP103 (Pspac-plsC amyE::Pdes-lacZ) cells grown ON at 37°C in Spizizen minimal medium supplemented with 0.2 mmol/L IPTG were washed and used to inoculate fresh media, in the presence (black bars) or absence (gray bars) of 1 mmol/L IPTG. At OD₅₂₅ of 0.35 cultures were split and incubated at 25 or 37°C. β-galactosidase specific activities were determined 4 h after the temperature shift. Values expressed in Miller units (MU) are representative of three independent experiments.