Table 1.
Bacterial strains and plasmids.
| Strain or plasmid | Relevant characteristics1 | Source or reference |
|---|---|---|
| Bacillus subtilis strains | ||
| JH642 | trpC2 pheA1 | Laboratory stock |
| AKP3 | JH642 amyE::Pdes–lacZ; Cmr | Aguilar et al. (2001) |
| CM21 | AKP3 desKR::Km, thrC::PxylA-desR; Kmr Spr Cmr | Albanesi et al. (2004) |
| GS77 | JH642 fabF1 (FabFI108F) cerulenin-resistant | Schujman et al. (1998) |
| BLUP87 | GS77 amyE::Pdes–lacZ; Cmr | This study |
| BLUP34 | JH642 plsC::PspacOid-plsC; Cmr | This study |
| BLUP102 | JH642 plsC::PspacOid-plsC; Spr | This study |
| BLUP103 | BLUP102 amyE::Pdes-lacZ; Cmr Spr | This study |
| Escherichia coli strain | ||
| DH5α | supE44 thi-1 ΔlacU169 (Φ80lacZΔM15) endA1 recA1hsdR17gyrA96 relA1trp-6 cysT329::lac inmpI(209) | Laboratory stock |
| Plasmids | ||
| pCR-BluntII-TOPO | pCR-Blunt II-TOPO E. coli cloning vector; Kmr | Invitrogen |
| pJM116 | Integrative vector to construct transcriptional fusions to lacZ; integrates at the amyE locus of B. subtilis; Cmr | Perego (1993) |
| pHPKS | B. subtilis replicative vector of low copy number, Ermr Lmr | Johansson and Hederstedt (1999) |
| pCm::Sp | Antibiotic switching vector; Ampr, Spr | Steinmetz and Richter (1994) |
| pDH88 | Integrative plasmid containing the IPTG-induciblePspac promoter; Cmr | Henner (1990) |
| pMUTIN4 | Integrative plasmid containing the IPTG-inducible Pspac-oid promoter; Ermr Lmr | Vagner et al. (1998) |
| pAR11 | Contains Pdes cloned into the EcoRI–BamHI sites of pJM116 | Aguilar et al. (2001) |
| pCM9 | PxylA-desKC cloned into pHPKS | Albanesi et al. (2004) |
| pLUP30 | PspacOid of pMUTIN4 cloned into the EcoRI–HindIII sites of pDH88 | This study |
| pLUP32 | 5´ end of the plsC gene cloned into the HindIII–SphI sites of pLUP30 | This study |
| pLUP124 | PxylA-desK cloned into pHPKS | This study |
1
Cmr, Spr, Kmr, Ermr, Lmr, and Ampr denote resistance to chloramphenicol, spectinomycin, kanamycin, erythromycin, lincomycin, and ampicillin, respectively.