Table 3.
Fatty acid composition of the phospholipids of a strain deficient in PlsC.
| Fatty acid | Percentage of fatty acid type1 |
|||||
|---|---|---|---|---|---|---|
| 25°C |
37°C |
|||||
| PlsC+ | PlsC− | ±2 | PlsC+ | PlsC− | ±2 | |
| Iso C13:0 | 0.20 | 0.29 | 0.26 | 0.14 | ||
| Anteiso C13:0 | 0.52 | 0.20 | ND | ND | ||
| Iso-C14:0 | 1.67 | 1.77 | 2.27 | 0.83 | ||
| n-C14:0 | 0.43 | 0.50 | 0.70 | 0.44 | ||
| Iso-C15:0 | 14.69 | 10.89 | 23.44 | 7.04 | ||
| Anteiso C15:0 | 38.18 | 27.33 | 30.84 | 11.08 | ||
| n-C15:0 | 1.38 | 1.11 | 1.63 | 0.92 | ||
| Iso-C16:1 | 1.66 | 1.73 | ND | 0.12 | ||
| Iso-C16:0 | 3.12 | 4.34 | 6.54 | 4.99 | ||
| n-C16:1 | 4.25 | 3.99 | 0.28 | 0.32 | ||
| n-C16:0 | 7.09 | 7.20 | 7.43 | 10.76 | ||
| Iso-C17:1 | 4.59 | 5.93 | ND | ND | ||
| Anteiso C17:1 | 1.28 | 2.84 | ND | ND | ||
| n-C17:0 | 0.67 | 1.24 | 0.74 | 12.88 | ||
| Iso-C18:0 | 0.07 | 0.77 | 0.62 | 3.09 | ||
| n-C18:1 | 1.83 | 3.64 | 2.30 | 15.90 | ||
| Iso-C19:0 | ND | 0.26 | 0.48 | 2.13 | ||
| Anteiso C19:0 | ND | 0.48 | 0.13 | 1.86 | ||
| n-C19:0 | ND | 0.16 | ND | 0.79 | ||
| Iso-C20:0 | ND | ND | ND | 1.12 | ||
| n-C20:0 | ND | 0.40 | ND | 3.73 | ||
| Iso-C21:0 | ND | ND | ND | 0.39 | ||
| Anteiso C21:0 | ND | ND | ND | 0.31 | ||
| n-C21:0 | ND | ND | ND | 0.13 | ||
| Iso-C22:1 | ND | ND | ND | 0.19 | ||
| Iso-C22:0 | ND | ND | ND | 0.25 | ||
| UFAs | 11.8 | 14.5 | 1.2 | 0.3 | 0.6 | 2.0 |
| LCFA/SCFA3 | 0.8 | 1.4 | 1.8 | 0.7 | 3.9 | 5.6 |
| Iso/anteiso4 | 0.6 | 0.8 | 1.3 | 1.1 | 1.4 | 1.3 |
| Straight-chain FA | 15.7 | 18.2 | 1.2 | 13.1 | 45.9 | 3.5 |
| Iso + straight/anteiso5 | 0.9 | 1.1 | 1.2 | 1.5 | 3.4 | 2.3 |
ND, not detected.
BLUP103 (Pspac-plsC) cells grown ON at 37°C in MM supplemented with 0.2 mmol/L IPTG were washed and used to inoculate fresh media, in the presence (PlsC+) or absence (PlsC−) of 1 mmol/L IPTG. At DO600 of 0.35 cultures were split and incubated at 25 or 37°C for 6 h. When growth stopped because of PlsC depletion, total lipids were extracted, transesterified to yield FA methylesters and subjected to GC-MS analysis.
Ratio of percentages of not induced cells compared to induced cells.
Ratio of long-chain FA (LCFA, C16 or longer) to short-chain FA (SCFA, C13 to C15).
Ratio of iso-branched-chain FA to anteiso-branched-chain FA.
Ratio of (iso-branched-chain FA + straight-chain FA) to anteiso-branched-chain FA.