Staphylococcus aureus |
RN4220 |
Mcs; restriction negative |
Novick (1991) |
COL |
Homogenous Mcr (MIC, 1600 μg mL−1); Ems
|
Rockefeller University Collection |
Escherichia coli |
DH5α
|
recA endA1 gyrA96 thi-1 hsdR17 supE44 relA1 ϕ80 ΔlacZΔM15 |
Invitrogen |
BL21(DE3) |
F–ompT gal dcm lon hsdSB(rB−mB−) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) |
Invitrogen |
Plasmids |
pET28a(+) |
E. coli expression vector |
Novagen |
pET-AMR1–3GL |
pET28a(+) expressing AMR1-3GL as a N-terminal His-tag fusion (fragment amplified with Pexp1 and Pexp4) |
This study |
pET-AMR1–2
|
pET28a(+) expressing AMR1-2 protein as a N-terminal His-tag fusion. pET-AMR1-3GL was used to insert a stop codon by directed mutagenesis with primers Pexp_stop1 and Pexp_stop2 |
This study |
pET-AM |
pET28a(+) expressing AM as a N-terminal His-tag fusion (fragment amplified with Pexp1 and Pexp5) |
This study |
pET-R3GL |
pET28a(+) expressing R3GL as a N-terminal His-tag fusion (fragment amplified with Pexp2 and Pexp4) |
This study |
pET-GL |
pET28a(+) expressing GL as a N-terminal His-tag fusion (fragment amplified with Pexp3 and Pexp4) |
This study |
pET-GLN
|
pET28a(+) expressing GLN as a N-terminal His-tag fusion (fragment amplified with Pexp3 and Pexp7) |
This study |
pET-GLC
|
pET28a(+) expressing GLC as a N-terminal His-tag fusion (fragment amplified with Pexp6 and Pexp4) |
This study |