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. 2014 Mar 4;3(2):257–270. doi: 10.1002/mbo3.166

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© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Role of ORF16 in phage S24-1 adsorption. The means and standard deviations are shown in the graphs (n = 6). The asterisks in the graphs indicate statistically significant differences (P < 0.01). (A) Phage-adsorption interference assay using rORF16. The white triangles and circles indicate the phage-adsorption efficiencies in the presence of BSA and ORF16, respectively. The black box indicates the phage-adsorption efficiency without any protein supplements (control). The phage-adsorption efficiencies were compared for the BSA and rORF16 treatments at the same protein concentrations. (B) Phage-adsorption interference assay using anti-ORF16 rabbit serum. The phage concentration was measured after incubation with diluted anti-ORF16 rabbit sera or preimmunized rabbit serum (30 min, 37°C). The untreated phage concentration was set at 100%.