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. 2014 Feb 6;5(3):764–774. doi: 10.18632/oncotarget.1769

Figure 4.

Figure 4

A, C4-2 invasion was quantified 24h after the start of the invasion assay. Cells were transfected with 2nM Negative Control (NC) or PCATT18-targeting siRNA1 and siRNA2. Columns represent mean value (4 experiments) bar SD. ***p<0.001 (ANOVA and Dunnett's post-test). B, C4-2 cell migration was quantified at 6h, 24h or 48h post-transfection, **P<0.01, ***P<0.001 (siRNA vs. NC), 2 way ANOVA and Tukey's post-test. C, D, E, MTT assay was performed on LNCaP (C) C4-2 (D) and BPH (E) cells treated with negative control (NC) or PCAT18-targeting siRNAs (both at 2nM concentration) on days 1-3-5 post-transfection, as previously described [30]. Dots represent mean value, lines standard deviation (2 experiments performed in triplicate, data normalized to cell number in NC-day1) ***p<0.001 (2 way ANOVA and Tukey's post-test). F, LNCaP cells were transfected with negative control (NC) or PCAT18-targeting siRNAs for 5 days. Bars represent mean values, lines standard deviations (2 independent experiments performed in triplicate). ***p<0.001 with respect to NC (ANOVA and Dunnet's post-test).