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293, 293T, and HepG2 cells were transfected with plasmids of p53-TA-Luc plus RSV-lacZ, and then incubated with ActD (10 nM) for the indicated time periods, followed by the activity assays of luciferase and β-galactosidase. Each experiment was assayed in triplicate and repeated at least three times. *** Indicates p < 0.001 compared with the negative controls.
