Skip to main content
. 2014 Feb 11;5(3):788–801. doi: 10.18632/oncotarget.1772

Figure 2. LIF promotes proliferation and anchorage-independent growth of breast cancer cells and promotes the growth of xenograft breast tumors.

Figure 2

(a) Ectopic LIF expression promoted proliferation of MCF7, T47D and MDA-MB-231 cells. Knockdown of endogenous LIF inhibited the growth of MDA-MB-231 cells. (b) Ectopic LIF expression promoted anchorage-independent growth in soft agar of breast cancer cells. Left panels: Representative images of colonies in soft agar. Right panels: average number of colonies (upper) and average area of colonies (lower). (c) Knockdown of LIF inhibited anchorage-independent growth in soft agar of MDA-MB-231 cells. (d) Ectopic LIF expression promoted the growth rate of xengoraft breast tumors. BALB/c nude mice were inoculated (s.c.) with MCF7-LIF, T47D-LIF, MDA-MB-231-LIF and their control cells (Con). Upper panels: Representative images of xenograft tumors; lower panels: growth curves of xenograft tumors. (e) Knockdown of LIF reduced the growth rate of MDA-MB-231 xenograft tumors. Data are presented as mean ± SD (n=3 for a-c; n=10 for d & e). *: p<0.001.