Skip to main content
. 2014 Feb 11;5(3):788–801. doi: 10.18632/oncotarget.1772

Figure 3. LIF activates the mTOR pathway in breast cancer cells.

Figure 3

(a) Exogenous LIF (100 ng/ml) increased the levels of phosphorylated p70S6K at Thr-389 (p-p70S6K) and phosphorylated 4EBP1 at Thr 37/46 (p-4EBP1) in MCF7, T47D and MDA-MB-231 cells. (b) Ectopic LIF expression in MCF7, T47D and MDA-MB-231 cells increased p-p70S6K and p-4EBP1 levels (left panels), whereas knockdown of LIF in MDA-MB-231 cells decreased p-p70S6K and p-4EBP1 levels (right panels). (c) Ectopic LIF expression increased p-p70S6K and p-4EBP1 levels in T47D and MDA-MB-231 xenograft tumors. Knockdown of LIF reduced p-p70S6K and p-4EBP1 levels in MDA-MB-231 xenograft tumors. The expression levels of LIF in xenograft tumors were confirmed by Western-blot assays.