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. 2014 Feb 11;5(3):788–801. doi: 10.18632/oncotarget.1772

Figure 6. Blocking the AKT signaling inhibits the promoting effect of LIF on tumorigenesis and metastasis in breast cancer cells.

Figure 6

(a) MCF7, T47D and MDA-MB-231 cells were treated with LIF (100 ng/ml) alone or in combination with wortmannin (2 μM), and the invasion and migration abilities of cells were determined. (b) MCF7-LIF, T47D-LIF, MDA-MB-231-LIF and their control cells were treated with or without wortmannin (2 μM), and the invasion and migration abilities of cells were determined. (c) Blocking AKT activity by the expression of DN-AKT largely abolished the effect of LIF on invasion and migration abilities of MCF7-LIF and T47D-LIF cells. (d) The expression of DN-AKT largely abolished the promoting effect of LIF on proliferation in MCF7-LIF and T47D-LIF cells. (e) The expression of DN-AKT largely blocked the promoting effect of LIF on anchorage-independent growth in soft agar in MCF7-LIF and T47D-LIF cells. (f) The expression of DN-AKT largely blocked the promoting effect of LIF on growth rate of xenograft T47D-LIF tumors. Data are presented as mean ± SD. n=3 for a–e; n=10 for f. Scale bar: 50 μM. *: p<0.001; #: p<0.01.