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. 2014 Feb 2;5(3):754–763. doi: 10.18632/oncotarget.1768

Figure 4. Impact of MUC1 expression on signaling pathways.

Figure 4

(A) Western blotting on whole cell extracts obtained from EV- and MUC1FL-ACHN clones or from 786-O scramble, sh1.1 and sh1.2 clones were performed. Antibodies against Bax, Bcl-xL, caspase 9, c-Myc, Akt, phospho-Akt, p38, phospho-p38, ERK, phospho-ERK, JNK, phospho-JNK, cyclin D1, β-catenin and β-actin were used. (B) Expression of NF-κB p65 and p50 subunits in cytosolic and nuclear extracts was carried out by western blot. (C-D) Luciferase activity of the κB-Luc synthetic promoter was measured 48h after transfection. Luciferase activity in EV-ACHN and scramble 786-O cells was set as 1. Values are means s.e.m and represent five separate experiments (*** p<0.001).