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. 2014 Feb 2;5(3):754–763. doi: 10.18632/oncotarget.1768

Figure 5. Increase of invasive properties mediated by nuclear MUC1-C is dependent of ADAM10/ADAM17/γ-secretase activities.

Figure 5

(A) Western blotting was performed on nuclear fraction of ACHN cells after treatment by different siRNA. The intensities of the signals were determined by densitometric scanning and are expressed as the relative signal intensity compared with that obtained with control siRNA. (B) Expression of MUC1-CT in cytosolic and nuclear fractions was carried out by western blotting from EV- and MUC1FL-ACHN clones treated or not with 10 ng/ml of L685,458, a γ-secretase inhibitor. (C) Cell invasion of ACHN cells transfected with different siRNA or treated 24h with 10 ng/ml of L685,458 was evaluated using 24-well Matrigel® invasion chambers with 10% fetal calf serum as chemoattractant. The graphs show the total number of invasive cells counted 24h after seeding. Values are means s.e.m and represent five separate experiments. (D) Invasion experiment was also performed on ACHN cells treated with 5 μM of CP-2 (control) or GO-203 peptides. Values are means s.e.m and represent five separate experiments (** p<0.01, *** p<0.001)