Table 1. Mutations in evolved OAR isolates from the fungal–bacterial community.
| Isolate | Gene |
Mutation |
|
|---|---|---|---|
| Change in nucleotide sequencea | Change in amino-acid sequence | ||
| OAR-A1 | DctA | Δ375–527 | In-frame deletion of 50 aa (Δ126–176) |
| M48-B12 | 1145 T>G | 382 M>R | |
| M48-A8 | C insertion at 92 | Truncation; retains first 31 native aa+10 nonsense aa added | |
| M48-B8 | T insertion at 88 | Truncation; retains first 29 native aa+12 nonsense aa added | |
| M72-F8 | G insertion at 96 | Truncation; retains first 33 native aa+8 nonsense aa added | |
| M72-F10 | G insertion at 92 | Truncation; retains first 30 native aa+11 nonsense aa added | |
| M72-G12 | ΔT, 84 | Truncation; retains first 94 native aa+11 nonsense aa added | |
| M72-E9 | DctB | ΔT, 380 | Truncation; retains first 126 native aa+103 nonsense aa added |
| M72-F12 | A insertion at 162 | Truncation; retains first 54 native aa+53 nonsense aa added | |
| M72-G1 | ΔG, 627 | Truncation; retains first 218 native aa+20 nonsense aa added | |
| M72-G6 | 352 G>T | 118V>F | |
| M72-H4 | ΔT, 380 | Truncation; retains first 218 native aa+20 nonsense aa added | |
| M72-H10 | 352 G>T | 118V>F | |
| M72-E3 | dctD | G insertion at 246 | Truncation; retains first 82 native aa+18 nonsense aa added |
| M72-F12 | ΔA, 227 | Truncation; retains first 75 native aa+3 nonsense aa added | |
| M48-B6 | rpoN1 | ΔT, 1409 | Truncation; retains first 469 native aa+9 nonsense aa added |
| M72-E8 | C insertion at 116 | Truncation; retains first 190 native aa+2 nonsense aa added | |
| M72-E10 | ΔA, 1507 | Truncation; retains first 502 native aa+40 nonsense aa added | |
| OA48-B11 | 34 A>T ΔA, 1507 | 12 S>C retains first 502 native aa+40 nonsense aa added | |
| FOAR-A6 | pyrE | G insertion at 130, 368 T>C | Truncation; retains first 43 native aa+64 nonsense aa added |
| FOAR-A7 | Δ74,75 | Truncation; retains first 25 native aa+81 nonsense aa added | |
| FOAR-C8 | Δ74,75 | Truncation; retains first 25 native aa+81 nonsense aa added | |
| FOAR-C11 | Δ74,75 | Truncation; retains first 25 native aa+81 nonsense aa added | |
| FOAR-C12 | Δ74,75 | Truncation; retains first 25 native aa+81 nonsense aa added | |
| FOAR-B6 | A insertion at 124 | Truncation; retains first 41 native aa+66 nonsense aa added | |
| FOAR-A9 | pyrF | 312 C>A 313 T>G 320 T>G | 105 S>A 107 L>R 108 C>G |
| FOAR-D5 | Δ15 | Truncation; retains first 4 native aa+2 nonsense aa added | |
| OA72-G4 | Δ248 | Truncation; retains first 82 native aa+3 nonsense aa added | |
| OA72-H7 | A insertion at 39 | Truncation; retains first 13 native aa+20 nonsense aa added | |
Abbreviations: aa, amino acids; OAR, OA-resistant. aNumbering of sequences is from the first nucleotide in the start codon.