Table 2. Metabolite content of cell-free filtrates from phage-infected Sulfitobacter sp. 2047 relative to control cultures at 480 min post infection.
| Metabolite | Fold changea | Metabolite | Fold changea |
|---|---|---|---|
| Amino Acids | Nucleic acids, nucleosides and nucleotides | ||
| Asparagine | 2.37(+) | Deoxyadenosineb | 4.69(+) |
| Methionineb | 1.83(+) | Thymineb | 2.62(+) |
| Cysteine | 1.74(+) | Cytosineb | 1.55(+) |
| Tryptophan | 0.73 | dCDP | 1.24 |
| Threonine | 0.10(−) | UDP | 1.12 |
| Homoserine | 1.87(+) | 5′-Methylthioadenosine | 1.05 |
| GABAb | 1.45 | Orotate | 0.95 |
| Betaine | 1.29 | Dihydrooroate | 0.87 |
| 1-Methylhistidine | 1.28 | N-acetylglucosamine-1-phosphate | 0.8 |
| O-Acetylserine | 0.91 | TDP | 0.57(−) |
| S-Adenosylmethionine | 0.88 | GMP | 0.42(−) |
| N-Acetylornithineb | 0.56(−) | 5-Methyldeoxycytidineb | 0.35(−) |
| TCA cycle | Pentose phosphate | ||
| Malonyl CoAc | 1000.00(+) | Fructose-1,6-bisphosphate | 0.99 |
| Succinyl CoA | 1.34 | Sedoheptulose-7-phosphate | 0.71 |
| Succinate | 0.78 | Erythrose-4-phosphate | 0.00(−) |
| (Fumarate, maleate and isoketovalerate)d | 0.72 | ||
| Citrate | 0.7 | Cofactors, vitamins and electron carriers | |
| 2-Oxoglutarateb | 0.62(−) | Acetyl CoA | 35.17(+) |
| 3-Phosphoglycerate | 1.89(+) | Pyridoxine | 4.63(+) |
| (1,3 and 2,3 bisphosphoglycerate) | 1.1 | Nicotinate | 0.95 |
| Phosphoenolpyruvate | 0.61(−) | 5-Methyltetrahydrofolate | 0.85 |
| Methylmalonic acid | 0.79 | ||
| Lipids | Thiamine | 0.29(−) | |
| (Phosphatidylcholine, phosphatidylethanolamine)c,d | 1000.00(+) | FAD | 0.00(−) |
| Propionyl CoA | 79.81(+) | NAD | 0.00(−) |
| Ethanolamine | 2.93(+) | ||
| Palmitate | 0.97 | Other | |
| Farnesylpyrophosphate | 0.57(−) | Urea | 2.78(+) |
| pipecolic acid | 1.89(+) | ||
| Acetyl phosphate | 0.92 | ||
| 4-Hydroxybenzoate | 0.81 | ||
| Citraconate | 0.42(−) | ||
| Phenylpyruvateb | 0.37(−) | ||
| Lactate | 0.32(−) |
Abbreviations: Co A, Coenzyme A; dCDP, deoxycytidine diphosphate; FAD, flavin adenine dinucleotide; GMP, guanosine monophosphate; NAD, nicotinamide adenine dinucleotide; TCA, tricarboxylic acid; TDP, thymidine diphosphate; UDP, uridine diphosphate.
a
Metabolite levels in infected cultures are expressed relative to levels in control cultures at 480 min. Elevated metabolites ((+); fold change ⩾1.5) and depressed metabolites ((−); fold change ⩽0.67).
b
Not detected in intact cells, detected in filtrates only.
c
Metabolite was not detected in control cultures, and therefore 1000 was used to denote a large increase in concentration in the infected cells.
d
Metabolites are indistinguishable with the applied method.