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. 2013 Dec 12;8(5):1008–1019. doi: 10.1038/ismej.2013.222

Figure 1.

Figure 1

16S rRNA pseudogenes present within the nuclei of T. agilis. (a) Agarose gel electrophoresis of 16S rRNA genes amplified by PCR with a bacteria-specific primer set from single nuclei samples of T. agilis. Lanes 1–6, PCR products from each of six nuclei. Lane 7 and 8, PCR products from single DNase-treated nuclei. N, negative control for PCR. (b) Structures of representative pseudogenes. Gray color denotes deleted regions. The positions of PCR primers specific to verrucomicrobia (Table 1) are indicated. RsTaN-K1 is an authentic 16S rRNA phylotype. RsTaN2-18 originated from RsTaN-K2 and the others shown here originated from RsTaN-K1. The positions correspond to those in Escherichia coli (J01695).