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. Author manuscript; available in PMC: 2014 Apr 23.
Published in final edited form as: Eur J Neurosci. 2011 Jul 4;34(3):362–373. doi: 10.1111/j.1460-9568.2011.07782.x

Figure 3.

Figure 3

Quantification of dendrite development in eGFP+ newborn neurons by Sholl analysis at 14, 28 and 42 DPI in WT and mutant mice. The number of intersections between eGFP+ dendrite segments and virtual concentric lines centered on the soma and spaced by 10 μm is displayed graphically. For statistical comparison between genotypes, the corresponding AUC was calculated in each graph (see Table 1 for mean values and statistical results). Representative examples of eGFP+ cells in each genotype are illustrated. (A) At 14 DPI, no difference in the number of intersections was observed between WT, α2- and δ-KO mice, whereas a significant reduction was seen in α4-KO mice, mainly proximally. (B) At 28 DPI, a significant reduction in the number of intersections was evident for both α2- and α4-KO mice compared to WT, whereas no difference was observed for δ-KO mice. (C) This alteration was maintained at 42 DPI, mainly due to loss of branches in newborn neurons from α2-KO mice. Scale bars, 20 μm.