Fig. 6.

Cholesterol efflux assay in THP-1, THP-1miR206 OE and THP-1miR206 KO cells. Control THP-1 cells, THP-1miR206 OE and THP-1miR206 KO were loaded with [³H]cholesterol for 24 h and cholesterol efflux to human serum, ApoB depleted serum (HDL), and ApoA-I was determined. a) Percentage of cholesterol efflux to ApoA-I, HDL and human serum was calculated relative to the unspecific cholesterol efflux to BSA. Statistical significance is indicated (t test: THP-1miR206 OE vs. control and THP-1miR206 OE vs. THP-1miR206 KO efflux to human serum; ^#p < 0.05; THP-1miR206 OE vs. THP-1miR206 KO efflux to HDL; ^$p < 0.05; efflux to ApoA-I, HDL and human serum compared to control-BSA; *p < 0.05; **p < 0.01; ***p < 0.001). b) Cholesterol efflux assay from WT and miR206KO MPMs to ApoA-I, HDL and whole human serum: MPMs were isolated and loaded with ³H-acLDL for 24 h and the cholesterol efflux assay was performed. Results are mean ± SEM of triplicate experiments. Statistical significance is indicated (t test; *p < 0.05; ***p < 0.001).