Skip to main content
. Author manuscript; available in PMC: 2014 Apr 23.
Published in final edited form as: J Immunol. 2008 Nov 1;181(9):6148–6157. doi: 10.4049/jimmunol.181.9.6148

FIGURE 4.

FIGURE 4

Expansion of activated CD8+ T cells releasing IFN-γ in the lungs of EBI-3-deficient mice in a murine model of melanoma. The total number of CD8+ T cells isolated from the lungs of EBI-3−/− and wild-type littermates did not differ (4.673 ± 0.63 and 5 ± 0.438 for wild-type and EBI-3−/− respectively). a, Lung CD8+ T cells from wild-type and EBI-3−/− mice were isolated at day 5 after tumor cell injection. Production of IFN-γ by anti-CD3 Ab stimulated CD8+ T cells was measured after 24 h by ELISA. CD8+ T cells from EBI-3(−/−) mice produced significantly (***, p < 0.001) higher levels of IFN-γ as compared with wild-type control cells. b, Lungs of EBI-3−/− and B6 wild-type mice were stained for the indicated markers and analyzed by FACS (upper panels). VLA-4 (CD49d) was found to be significantly increased on activated EBI-3−/− CD8+ T cells after B16-F10 cell injection (lower panels). Lung CD8+CD122+ T cells were down-regulated in EBI-3−/− mice 5 days after i.v. injection of the B16-F10 cell line. c, Increase of lung CD8+ T cells in EBI-3−/− mice during tumor development as compared with wild-type littermates as shown by FACS analysis. All data are representative of three independent experiments (five mice per group).