Table 2.
The primers for clone sequencing
| Primer name | Forward primer | Reverse primer | Amplicon size (bp) |
|---|---|---|---|
| FH1 |
GGAGTTTTTTATGTATTTGGTATTT |
CATACCCCCAAAAAATAAAATTTAAAATCT |
383 |
| FH2 |
ATGGGGAAGTAGATTTGGGTAT |
TCACTTTAACTACCCCCAAATATTATAA |
456 |
| FH3 |
TAGGGGTGTTATGAAGGTGGTA |
ATTCTATATAATCACTTCCACATTCTTAAT |
418 |
| FH4 |
AGGGTTGGGTAATATTTTTATTGTTATAAT |
ACAAACCAAATTACCCCCATT |
492 |
| FH5 |
ATGGGAGGTGGGTTTTGTT |
AAAATCACCTACCAACCTCAATAACTA |
446 |
| FH6 |
GGTTTTTTAGATTTGGATGTGATTTAGG |
CCCCCCCATAAATAAAACCTT |
470 |
| FH7 |
GTGGAGATAGTAGTGAGTAGAGA |
AATCCCATATAAACCCAAACCT |
484 |
| FH8 |
TTTTTTTTGTAGTTTAGGGTTTTTTGTAT |
CTCTTCTAAAACATCCACCTTACA |
391 |
| FH9 |
TGAGAAATTTAAGGAGTGTGTGT |
TACCCCCATCTATCCTATACTCT |
452 |
| FL1 |
GGGAGGAAGGAGTTGGTAGGTTTTTAT |
ACTACCCAAATCTCTCACTTTACAATAAC |
357 |
| FL2 |
GTTTTTAGTTAAGTTTTTTGGGGAGAGG |
AACAACTAATTTCACACCATATCTTAT |
349 |
| FL3 |
TGGGAAGTATGTGGAAGTGGA |
CACCCCCCATTTTAAAAACAACAATCTAT |
290 |
| FL4 |
GGAAATGTGTTAAGGTAAGAGGTTATAAG |
CCAAAACTTCTACCCCTAAAAAT |
452 |
| FL5 |
TTTTGTTAAGAATAATAAGAGAGGGTAAGT |
ATCTATAAATCTTTTTTCCAAACTCAC |
481 |
| FL6 |
GGGTTTTTTGTTTAATTGTGTATTGGAG |
ACCTAACCAAACCCCATACT |
388 |
| FL7 |
GGATTATTAGTTGTATAATTATGGAGATT |
ACCCCCTCTCTACTACTAATATAA |
378 |
| FL8 |
AAGGGGATTTTTTTGGAAAGTAT |
CATCTCAAACTCTCACCATCATAAATA |
489 |
| FL9 |
GTGTTTAGTAATAGGTAGTTTGGAAAATT |
CTTACTTACAATTCCAATACCTATAATCAC |
435 |
| FL10 |
TGGTATTAGTGGGAGTTTAATAGATG |
AACTCAAATAAACCCACATACACTATAAT |
413 |
| FL11 |
TGGATTGATATAGGGAAAAAAAAGGAGAA |
CACTCCCAAATCTCTACTAACCTCTA |
465 |
| FL12 | GTGGGTTTTATTTAGGTTGTGAGTA | CTTAAACTCCTCTTCCCCAACAC | 350 |
FH indicates fetus hypermethylation DNA markers and FL indicates fetus hypomethylation DNA markers.