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. Author manuscript; available in PMC: 2015 Mar 6.
Published in final edited form as: Mol Cell. 2014 Feb 20;53(5):689–699. doi: 10.1016/j.molcel.2014.01.012

Figure 2. PolyP is a Protein-Protective Chaperone In Vivo.

Figure 2

(A) and (B) Wild-type (black) and Δppk (red) E. coli were grown to log phase, then treated with 0.4 mM HOCl. Expression of (A) the heat shock genes ibpA, hslO, dnaK, rpoH, or

(B) the DNA damage indicator gene sulA was measured by qRT-PCR (mean ± SD). See also Figure S2A.

(C) Insoluble protein fractions from exponentially-growing E. coli wild-type and mutant strains before and after addition of 1 mM HOCl.

(D) Survival of (left panels) and insoluble protein fractions from (right panels) exponentially-growing E. coli rpoH− strains before and after a shift from 30° to 46°C.

(E) E. coli strains containing no polyP (red squares), wild-type (black circles), or higher than normal levels of polyP (blue triangles) were grown to log phase, serially diluted, spot-titered on agar containing different concentrations of spectinomycin and scored for growth (mean ± SD). See also Figures S2B and S2C.