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. Author manuscript; available in PMC: 2014 Apr 23.
Published in final edited form as: Biotechniques. 2014 Jan;56(1):18–27. doi: 10.2144/000114123

Figure 3. Analysis of spatiotemporal dynamics of yeast colonies extracted from time course data.

Figure 3

(A) Examples of the features mean intensity and energy during a time-lapse measurement; green lines are for three replicates of the complex F29 strain and blue for the smooth strain YO779. (B) Snapshots of colonies of F29 (top) and YO779 (bottom) at three times during development (indicated by bold vertical lines in (A). (C) Dimensionality reduction of time-lapse feature trajectories using principal component analysis. The trajectories shown are for strain summaries, which are obtained by taking median across all individual colonies at each time point. Strains F29 and F45 are fluffy, while strains S1 (FY4, Table 1) and S2 (YO779, Table 1) are smooth.