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A/B) Structure of wt-tHisF (PDB number 1THF[21a]); colored residues are positions 199 (blue), 50 (orange), and 176 (red). C) HR-ESI-MS of wt-tHisF, tHisF-Az50, and tHisF-Az50-RhBCN. D) Fluorescence spectra (290 nm) of wt-tHisF, tHisF-Az50 (in buffer, 60% ACN, 6M GdmCl), and tHisF-Az50-RhBCN (ACN = acetonitrile; GdmCl = guanidinium chloride).
