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. Author manuscript; available in PMC: 2015 Jan 24.
Published in final edited form as: Chembiochem. 2013 Dec 20;15(2):223–227. doi: 10.1002/cbic.201300661

Table 2.

FRET data for dual-labeled scaffolds

Mutant (+Az50) Energy transfer Distance (r) in Å
Intensity Lifetime Intensity Lifetime
D174C (top) 0.54 0.63 58 55
K243C (bottom) 0.11 0.15 88 80