Figure 5.

Expression of left/right asymmetry markers during P. miniata embryonic development. Line 1) Nodal is first expressed in the ectoderm at the blastula stage. A second expression domain of Nodal appears in mid-gastrula stage embryos when it is expressed symmetrically in the developing archenteron. Nodal expression becomes restricted to the right side of the archenteron in late-gastrula stage embryos. Line 2) Lefty is first expressed in the ectoderm at the blastula stage. A second expression domain of Lefty appears in late-gastrula stage embryos when it is expressed asymmetrically in the right side of the archenteron. Line 3) Pitx2 is first expressed ubiquitously in oocytes. Pitx2 expression localizes to the right coelomic pouch and right ectoderm in late gastrula staged embryos stage. Pitx2 expression in the right coelomic pouch and right ectoderm persists through late larval stages. A sequence for the Neomycin (Neo) resistance gene (Line 4) was used as a negative control for the hybridization procedure. Asterisks (*) show areas of emphasis for transcriptdetection. Dorsal views of the larva. Scale bar represents 100 μm.