FIG. 2.

Effect of increased adipocyte number on primary stromal cell support of primitive hematopoietic cells. BM MNCs were grown as stromal layers in the presence of 10 μM troglitazone or control dimethyl sulfoxide (DMSO). (A) Quantification of the total adipocyte concentration following solubilization of the Oil Red-O dye. (B) CAFC frequency of BM MNCs co-cultured on stromal cell layers grown in the presence of troglitazone or control conditions. Five independent experiments are shown, with the mean represented as the solid line. (C) CAFC frequency of BM MNCs co-cultured on stromal cell layers grown in the presence of troglitazone or control conditions with added neutralizing anti-adiponectin antibody. Two independent experiments are shown. *P<0.05; **P<0.001.