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. 2014 Apr 2;2014:379847. doi: 10.1155/2014/379847

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Copyright © 2014 J. Glaser et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

(a) Relative CapG expression of ovarian cancer cell lines SK-OV-3 and Hey, prior to and after alteration of CapG expression using CapG mediated siRNA (SK-OV-3 + siCapG) and stable retroviral transfection (Hey CapG), respectively. Values were calculated performing qRT-PCR in duplicates, repeated three times. Each probe was normalized to the housekeeping gene GAPDH. (b) Effects were confirmed by Western blot analyses. Equal detection of α-tubulin in samples verified equality of protein quantity. (c, d) Performing a migration assay (scratch assay), the motility in CapG siRNA treated cells was nearly halved in 24 hrs while overexpression in Hey cells led to a significantly earlier closure of the scratch after 72 hrs. (e, f) The invasiveness of siRNA treated cells was also significantly decreased in the transwell Matrigel invasion assay (by 4-fold) in SK-OV-3 cells.