FIG. 1.

Expression patterns of the uncoupling protein 2 (UCP2) fusion protein in Hepa 1–6 cells. (A) Green fluorescent protein (GFP) and fluorescent UCP2 immunocytochemistry (IC) signals of Hepa 1–6 cells transfected with either C-sense UCP2 or noncoding UCP2 show a UCP2 expression pattern in hepatocytes. GFP+IC shows merged pictures of GFP and immunostaining. (B) Western blot analysis of total proteins from transfected Hepa 1–6 cells using alkaline phosphatase–labeled anti-GFP antibody (Clontech, 1:2000 dilution). The figure shows ∼60-kD protein bands in sense UCP2-transfected cells, and ∼30-kD protein bands in noncoding UCP2 and vector-transfected cells. Western blot samples from left to right: transfections with C-UCP2, N-UCP2, noncoding-UCP2, and vector alone, respectively. Color images available online at www.liebertpub.com/met