FIG. 4.

The inhibitory effect of uncoupling protein 2 (UCP2) expression on the cell cycle is enhanced by genistein treatment. This figure shows cell populations in G₁, S, and G₂ phases in green fluorescent protein (GFP)-positive transfected cells (left) and GFP-negative nontransfected cells from the same transfection preparations: (1) C-UCP2-transfected cells; (2) C-UCP2-transfected cells with 24 hr of treatment with 200 μM genistein; (3) C-UCP2-transfected cells with 24 hr of treatment with okadaic acid; (4) noncoding UCP2-transfected cells; (5) vector alone–transfected cells. Data represent three repeated experiments. (*) Statistical analysis versus noncoding and/or vector alone–transfected cells (as indicated); (#) statistical analysis versus C-UCP2 transfected cells. (**) or (##) P<0.01.