FIG. 5.

CDK6 is decreased in uncoupling protein 2 (UCP2)-transfected cells. Western blot analysis of cell division–related proteins from total cellular protein preparations of Hepa 1–6 cells 48 hr after transfection shows a remarkable decrease of Cdk6 but not Cdk4; cyclins D1 and D3 remain constant. Mouse monoclonal antibodies against cyclin D1 (1:1000 dilution); cyclin D3 (1:1000 dilution), Cdk4 (1:2000 dilution), Cdk6 (1:1000 dilution) were all from cell signaling, and anti-β-actin antibody (1:2000 dilution) was from Sigma.