Table 1.
Effect of Uncoupling Protein 2 (UCP2) Expression on the Cell Cycle in Hela, MG63, and 293 Cells
| G1 phase % in | S phase % in | G2-M phase % in | |||||
|---|---|---|---|---|---|---|---|
| Cell type | UCP2 | GFP+ cells | GFP− cells | GFP+ cells | GFP− cells | GFP+ cells | GFP− cells |
| Hela | Sense | 95.6% | 58.7% | 2.2% | 28.8% | 2.2% | 12.5% |
| Noncoding | 86.7% | 61.4% | 13.3% | 26.2% | 0.0% | 12.4% | |
| MG63 | Sense | 84.39% | 68.19% | 11.01% | 19.88% | 4.60% | 11.92% |
| Noncoding | 65.43% | 63.39% | 21.01% | 21.23% | 13.56% | 15.37% | |
| 293 cell | Sense | 67.81% | 50.05% | 19.24% | 32.93% | 12.95% | 17.01% |
| Noncoding | 41.33% | 44.30% | 37.18% | 36.97% | 21.50% | 18.73% | |
Forty-eight hours after transfection with sense or noncoding mouse UCP2 constructs, cell proliferation activity was analyzed by flow cytometry.
GFP, green fluorescent protein.