FIG. 3.

Glial scar evolution and axon remodeling 2 weeks post-injury; double immunofluorescence labeling for glial fibrillary acidic protein (GFAP) (A,B, E, F; green on merge image) and phosphorylated microtubule associated protein 1B (MAP1B-P) (C, D, E-F; red on merge image) on spinal cord sections of untreated, and chondroitinase ABC (ChABC)-treated animals. E and F are higher magnification merge images of insets in A,C and B,D, respectively). Note that ChABC treatment renders the glial scar less dense (compare A with B), and considerably increases the total number of axons in the injury site, as well as the percentage of axons properly oriented in the rostrocaudal direction (compare C with D). Bar: A-F, 130 μm; G,H, 25 μm. Color image is available online at www.liebertpub.com/neu