Figure 9.

Validation of aged Tg4–42 deep sequencing results through quantitative real-time polymerase chain reaction (qRT-PCR) analysis. To confirm the deep sequencing data, qRT-PCR experiments for various genes were performed on aged Tg4–42 and age-matched WT mice. Expression levels of Tg4–42 mice were compared to age-matched WT animals (dashed red line represents WT standard). Normalization was performed against the housekeeping gene β-Actin. ***p < 0.001; **p < 0.01; *p < 0.05; m age in months; n = 4–5 per group.