Table 5.
List of differentially expressed transcripts in aged 5XFAD mice.
| ID | Gene name | Gene description | GO biological process annotation/functions | log2 Fold change | Adjusted p-value |
|---|---|---|---|---|---|
| MGI:88228 | C4b | Complement component 4B | Endopeptidase inhibitor activity | 3.31 | 2.44E-27 |
| Inflammatory response | |||||
| Complement activation | |||||
| Immune response | |||||
| MGI:88225 | C1qc | Complement component 1, q subcomponent, C chain | Complement activation | 3.05 | 1.84E-23 |
| Immune response | |||||
| MGI:88223 | C1qa | Complement component 1, q subcomponent, alpha polypeptide | Phosphate transport | 2.99 | 1.31E-24 |
| Complement activation | |||||
| Immune response | |||||
| MGI:107341 | Ctss | Cathepsin S | Cysteine-type peptidase activity | 2.75 | 5.00E-21 |
| Hydrolase activity | |||||
| Proteolysis | |||||
| Immune response | |||||
| MGI:88224 | C1qb | Complement component 1, q subcomponent, beta polypeptide | Phosphate transport | 2.72 | 2.22E-19 |
| Complement activation | |||||
| Immune response | |||||
| MGI:1891190 | Ctsz | Cathepsin Z | Cysteine-type peptidase activity | 2.49 | 1.01E-15 |
| Hydrolase activity | |||||
| MGI:98932 | Vim | Vimentin | Structural constituent of cytoskeleton | 2.27 | 8.36E-13 |
| Identical protein binding | |||||
| Apoptotic process | |||||
| MGI:96074 | Hexb | Hexosaminidase B | Cation binding | 2.16 | 9.49E-15 |
| Protein homodimerization activity | |||||
| Beta-N-acetylhexosaminidase activity | |||||
| Protein heterodimerization activity | |||||
| MGI:88562 | Ctsd | Cathepsin D | Aspartic-type endopeptidase activity | 2.13 | 1.66E-16 |
| Hydrolase activity | |||||
| MGI:2148181 | Snora68 | Small nucleolar RNA, H/ACA box 68 | Non-coding RNA | 2.11 | 4.37E-14 |
| Uridine modifications | |||||
| MGI:87994 | Aldoa | Aldolase A, fructose-bisphosphate | Actin binding | 2.03 | 1.32E-15 |
| Fructose-bisphosphate aldolase activity | |||||
| Cytoskeletal protein binding | |||||
| Tubulin binding | |||||
| Glycolysis | |||||
| MGI:88127 | B2m | Beta-2 microglobulin | MHC class I receptor activity | 1.86 | 5.03E-10 |
| Cellular defense response | |||||
| Innate immune response | |||||
| MGI:108046 | Laptm5 | Lysosomal-associated protein transmembrane 5 | Transmembrane transport | 1.86 | 1.32E-07 |
| MGI:1333815 | Cx3cr1 | Chemokine (C-X3-C motif) receptor 1 | Chemokine receptor activity | 1.66 | 9.43E-07 |
| G-protein coupled receptor activity | |||||
| Transmembrane protein | |||||
| Signal transduction | |||||
| MGI:99554 | Lgals3bp | Lectin, galactoside-binding, soluble, 3 binding protein | Scavenger receptor activity | 1.66 | 1.19E-05 |
| Isomerase activity | |||||
| Cellular defense response | |||||
| Signal transduction | |||||
| MGI:1921298 | 4632428N05Rik | RIKEN cDNA 4632428N05 gene | Receptor activity | 1.63 | 1.48E-06 |
| MGI:96614 | Itgb5 | Integrin beta 5 | Integrin binding | 1.56 | 1.05E-06 |
| Receptor activity | |||||
| Cell adhesion | |||||
| MGI:1914877 | Olfml3 | Olfactomedin-like 3 | Scaffold protein | 1.53 | 1.36E-04 |
| MGI:96073 | Hexa | Hexosaminidase A | Beta-N-acetylhexosaminidase activity | 1.51 | 3.85E-06 |
| Protein heterodimerization activity | |||||
| Hydrolase activity | |||||
| MGI:1339758 | Csf1r | Colony -stimulating factor 1 receptor | Macrophage colony-stimulating factor Receptor activity | 1.45 | 1.58E-06 |
| Protein homodimerization activity | |||||
| ATP-binding | |||||
| Immune response | |||||
| MGI:107387 | Aqp4 | Aquaporin 4 | Porin activity | 1.42 | 8.90E-07 |
| Water transmembrane transporter activity | |||||
| MGI:1918089 | P2ry12 | Purinergic receptor P2Y, G-protein coupled 12 | ADP receptor activity | 1.40 | 2.68E-04 |
| Guanyl-nucleotide exchange factor activity | |||||
| G-protein coupled adenosine receptor activity | |||||
| Signal transducer activity | |||||
| MGI:1278340 | Rpl21 | Ribosomal protein L21 | Structural constituent of ribosome | 1.37 | 2.20E-04 |
| RNA binding | |||||
| MGI:107286 | Man2b1 | Mannosidase 2, alpha B1 | Carbohydrate binding | 1.35 | 4.77E-05 |
| Alpha-mannosidase activity | |||||
| Hydrolase activity | |||||
| MGI:1096881 | Eef1a1 | Eukaryotic translation elongation factor 1 alpha 1 | GTPase activity | 1.32 | 6.22E-06 |
| Translation elongation factor activity | |||||
| Regulation of transcription | |||||
| MGI:1915213 | Npc2 | Niemann Pick type C2 | Cholesterol binding | 1.27 | 1.20E-03 |
| Enzyme binding | |||||
| MGI:88561 | Ctsb | Cathepsin B | Cysteine-type peptidase activity | 1.25 | 1.21E-05 |
| Hydrolase activity | |||||
| Immune response | |||||
| MGI:88564 | Ctsl | Cathepsin L | Cysteine-type peptidase activity | 1.23 | 3.42E-04 |
| Hydrolase activity | |||||
| Histone binding | |||||
| Immune response | |||||
| MGI:1934664 | Rpph1 | Ribonuclease P RNA component H1 | Endoribonuclease activity | 1.23 | 1.21E-05 |
| MGI:1920174 | Anln | Anillin | Actin binding | 1.22 | 1.53E-03 |
| Phospholipid binding | |||||
| MGI:95832 | Grn | Granulin | Growth factor activity | 1.21 | 1.00E-03 |
| Cytokine activity | |||||
| Signal transduction | |||||
| MGI:98729 | Tgfbr2 | Transforming growth factor, beta receptor II | ATP-binding | 1.18 | 4.94E-03 |
| Transmembrane receptor protein serine/threonine kinase activity | |||||
| Transferase activity | |||||
| Receptor activity | |||||
| SMAD binding | |||||
| Signal transduction | |||||
| MGI:894320 | Prdx6 | Peroxiredoxin 6 | Glutathione peroxidase activity | 1.17 | 2.21E-04 |
| Oxidoreductase activity | |||||
| Antioxidant activity | |||||
| Response to oxidative stress | |||||
| MGI:1921305 | Plce1 | Phospholipase C, epsilon 1 | Guanyl-nucleotide exchange factor activity | 1.15 | 5.42E-03 |
| Calcium ion binding | |||||
| Receptor signaling protein activity | |||||
| Hydrolase activity | |||||
| Signal transducer activity | |||||
| MGI:107357 | Inpp5d | Inositol polyphosphate-5-phosphatase D | SH3 domain binding | 1.14 | 1.96E-02 |
| PTB domain binding | |||||
| Hydrolase activity | |||||
| Signal transducer activity | |||||
| Immune response | |||||
| MGI:1330838 | Lgmn | Legumain | Cysteine-type endopeptidase activity | 1.07 | 1.82E-03 |
| Peptidase activity | |||||
| Immune response | |||||
| Hydrolase activity | |||||
| MGI:1917329 | Golm1 | Golgi membrane protein 1 | Protein modification | 1.03 | 2.85E-02 |
| Nucleus organization | |||||
| MGI:88385 | Cfh | Complement component factor h | Heparin binding | 1.02 | 4.65E-02 |
| Heparan sulfate proteoglycan binding | |||||
| Complement activation | |||||
| Immune response | |||||
| MGI:95640 | Gapdh | Glyceraldehyde-3-phosphate dehydrogenase | Microtubule binding | 1.01 | 3.07E-03 |
| NADP binding | |||||
| MGI:1924096 | Rps9 | Ribosomal protein S9 | Structural constituent of ribosome | 1.01 | 1.59E-02 |
| RNA binding | |||||
| Translation regulator activity | |||||
| MGI:97171 | Mt1 | Metallothionein 1 | Organic cyclic compound binding | 1.01 | 3.08E-03 |
| Hormone binding | |||||
| Copper ion binding | |||||
| MGI:97591 | Pkm | Pyruvate kinase, muscle | Magnesium ion binding | 1.00 | 1.56E-03 |
| Pyruvate kinase activity | |||||
| ATP-binding | |||||
| MGI:88423 | Clu | Clusterin | ATPase activity | 0.96 | 2.50E-03 |
| Ubiquitin -protein ligase binding | |||||
| Misfolded protein binding | |||||
| Immune response | |||||
| MGI:1338892 | Padi2 | Peptidyl arginine deiminase, type II | Protein-arginine deiminase activity | 0.95 | 4.86E-02 |
| Calcium ion binding | |||||
| Hydrolase activity | |||||
| Immune response | |||||
| MGI:1915472 | Tubb4b | Tubulin, beta 4B class IVB | Structural molecule activity | 0.93 | 2.03E-02 |
| GTPase activity | |||||
| Double-stranded RNA binding | |||||
| Structural constituent of cytoskeleton | |||||
| Unfolded protein binding | |||||
| MGI:2445114 | Pisd | Phosphatidylserine decarboxylase | Lyase activity | 0.93 | 1.94E-02 |
| MGI:96247 | Hsp90ab1 | Heat shock protein 90 alpha (cytosolic), class B member 1 | Unfolded protein binding | 0.90 | 6.13E-03 |
| GTP binding | |||||
| ATP-binding | |||||
| Double-stranded RNA binding | |||||
| Ion channel binding | |||||
| Immune response | |||||
| Negative regulation of neuron apoptotic process | |||||
| MGI:1925017 | Ermn | Ermin, ERM-like protein | Actin filament binding | 0.90 | 3.02E-02 |
| MGI:105959 | Cox8a | Cytochrome-c oxidase subunit VIIIa | Cytochrome-c oxidase activity | 0.88 | 1.52E-02 |
| MGI:96748 | Lamp2 | Lysosomal-associated membrane protein 2 | Membrane glycoprotein | 0.88 | 2.94E-02 |
| TRNA ligase activity | |||||
| ATP-binding | |||||
| Hemostasis | |||||
| MGI:97748 | Ctsa | Cathepsin A | Enzyme activator activity | 0.86 | 3.15E-02 |
| Serine-type carboxypeptidase activity | |||||
| Hydrolase activity | |||||
| MGI:103099 | Cox6a1 | Cytochrome-c oxidase subunit VIa polypeptide 1 | Cytochrome-c oxidase activity | 0.85 | 2.02E-02 |
| MGI:1346074 | Fxr2 | Fragile × X mental retardation, autosomal homolog 2 | RNA binding | 0.84 | 3.11E-02 |
| Identical protein binding | |||||
| MGI:99607 | Abca1 | ATP-binding cassette, sub-family A (ABC1), member 1 | Apolipoprotein binding | 0.82 | 4.37E-02 |
| Phospholipid binding | |||||
| Cholesterol binding | |||||
| ATP-binding | |||||
| MGI:88252 | Calr | Calreticulin | Iron ion binding | 0.81 | 3.02E-02 |
| Calcium ion binding | |||||
| Hormone binding | |||||
| mRNA binding | |||||
| Regulation of transcription | |||||
| Signal transduction | |||||
| Immune system | |||||
| MGI:98467 | Syp | Synaptophysin | Transporter activity | 0.79 | 2.74E-02 |
| Calcium ion binding | |||||
| Cholesterol binding | |||||
| Syntaxin-1 binding | |||||
| SH2 domain binding | |||||
| Synaptic vesicle maturation | |||||
| Synaptic transmission | |||||
| MGI:98373 | Sparc | Secreted acidic cysteine rich glycoprotein | Extracellular matrix binding | 0.78 | 4.37E-02 |
| Calcium ion binding | |||||
| Signal transduction | |||||
| Hemostasis | |||||
| MGI:1096398 | Cd81 | CD81 antigen | MHC class II protein complex binding | 0.77 | 4.93E-02 |
| Regulation of immune response | |||||
| MGI:1915347 | Dynll2 | Dynein light chain LC8-type 2 | Cytoskeletal protein binding | −0.86 | 2.10E-02 |
| Motor activity | |||||
| MGI:1277955 | Bsn | Bassoon | Metal ion binding | −0.87 | 1.10E-02 |
| Synaptic transmission | |||||
| MGI:3648294 | Tnrc18 | Trinucleotide repeat containing 18 | DNA binding | −0.87 | 3.34E-02 |
| MGI:2145310 | Rnf44 | Ring finger protein 44 | Zinc ion binding | −0.89 | 3.49E-02 |
| MGI:103291 | Rai1 | Retinoic acid induced 1 | Zinc ion binding | −0.90 | 2.82E-02 |
| DNA binding | |||||
| Transcription factor | |||||
| MGI:1096362 | Nrxn2 | Neurexin II | Cell adhesion molecule binding | −0.90 | 1.19E-02 |
| Calcium channel regulator activity | |||||
| Metal ion binding | |||||
| Synaptic transmission | |||||
| MGI:1337000 | Rn45s | 45S pre-ribosomal 5 | Non-coding RNA | −0.90 | 5.41E-03 |
| MGI:88106 | Atp1a2 | ATPase, Na+/K+ transporting, alpha 2 polypeptide | Sodium:potassium-exchanging ATPase activity | −0.90 | 5.39E-03 |
| Metal ion binding | |||||
| Hydrolase activity | |||||
| ATP-binding | |||||
| MGI:96667 | Kcnc1 | Potassium voltage -gated channel, shaw-related sub-family, member 1 | Rectifier potassium channel activity | −0.92 | 1.07E-02 |
| Voltage-gated ion channel activity | |||||
| Synaptic transmission | |||||
| MGI:1347488 | Foxk1 | Forkhead box K1 | Transcription regulation | −0.94 | 3.02E-02 |
| Cell differentiation | |||||
| DNA binding | |||||
| Mg-ion binding | |||||
| MGI:106190 | Bcl11a | B cell CLL/lymphoma 11A (zinc finger protein) | B cell differentiation | −0.96 | 3.73E-02 |
| T cell differentiation | |||||
| Regulation of transcription | |||||
| MGI:1321395 | Ltbp4 | Latent transforming growth factor beta binding protein 4 | Growth factor binding | −0.96 | 4.59E-02 |
| Hormone secretion | |||||
| Regulation of cell differentiation | |||||
| MGI:2176606 | Scrt1 | Scratch homolog 1, zinc finger protein | Transcription regulation | −0.96 | 2.09E-02 |
| MGI:1925589 | Ttyh3 | Tweety homolog 3 | Chloride channel activity | −0.96 | 7.91E-03 |
| Transmembrane transport | |||||
| MGI:2444218 | Ahdc1 | AT hook, DNA binding motif, containing 1 | DNA binding | −0.97 | 1.84E-02 |
| MGI:98460 | Syn1 | Synapsin I | Neurotransmitter secretion | −0.98 | 3.98E-03 |
| Synaptic vesicle transport | |||||
| MGI:95617 | Gabra5 | Gamma-aminobutyric acid (GABA) A receptor, subunit alpha 5 | GABA-receptor | −0.99 | 1.52E-02 |
| Chloride transport | |||||
| Gamma-aminobutyric acid signaling pathway | |||||
| MGI:2143099 | AI593442 | Expressed sequence AI593442 | Unclassified | −1.00 | 4.30E-03 |
| MGI:1346031 | Tshz1 | Teashirt zinc finger family member 1 | Transcription factor | −1.00 | 1.73E-02 |
| DNA binding | |||||
| MGI:2444817 | C530008 M17Rik | RIKEN cDNA C530008M17 gene | Unknown | −1.00 | 2.26E-02 |
| MGI:2441680 | Tmem8b | Transmembrane protein 8B | Cell cycle regulation | −1.00 | 2.27E-02 |
| Cell matrix adhesion | |||||
| MGI:109169 | Epas1 | Endothelial PAS domain protein 1 | Regulation of transcription | −1.01 | 6.32E-03 |
| Angiogenesis | |||||
| Transcription | |||||
| Signal transduction | |||||
| Cellular stress response | |||||
| MGI:1351323 | Snord33 | Small nucleolar RNA, C/D box 33 | Unknown | −1.04 | 4.35E-02 |
| MGI:107363 | Stxbp1 | Syntaxin binding protein 1 | Release of neurotransmitters via syntaxin regulation | −1.04 | 7.75E-04 |
| Vesicle transport | |||||
| Exocytosis | |||||
| Regulation of insulin secretion | |||||
| MGI:2443847 | Sdk2 | Sidekick homolog 2 | Chemotaxis | −1.04 | 2.43E-02 |
| Protein targeting | |||||
| Cell adhesion | |||||
| MGI:1919559 | Tmem158 | Transmembrane protein 158 | Ras pathway | −1.06 | 2.27E-02 |
| MGI:102858 | Fosl2 | Fos-like antigen 2 | Regulation of transcription | −1.10 | 1.07E-02 |
| Cell regulation | |||||
| Fibroblasten proliferation | |||||
| MGI:2686934 | Zfhx2 | Zinc finger homeobox 2 | DNA binding | −1.11 | 6.32E-03 |
| Transcriptional factor activity | |||||
| MGI:96434 | Igf2 | Insulin-like growth factor 2 | Hormone activity | −1.11 | 4.47E-03 |
| Growth factor activity | |||||
| Cell proliferation | |||||
| Regulation of cell cycle | |||||
| Protein metabolism | |||||
| Hemostasis | |||||
| Signal transduction | |||||
| MGI:2444034 | 9530091C08Rik | RIKEN cDNA 9530091C08 gene | Unclassified non-coding RNA gene | −1.14 | 1.10E-02 |
| MGI:1313277 | Vamp2 | Vesicle-associated membrane protein 2 | Vesicle mediate transport | −1.14 | 2.79E-04 |
| Synaptic vesicle exocytosis | |||||
| Regulation of insulin secretion | |||||
| MGI:1890616 | Scube1 | Signal peptide, CUB domain, EGF-like 1 | Inflammatory response | −1.19 | 1.28E-03 |
| Endothelial cell differentiation | |||||
| MGI:2444210 | Nr1d1 | Nuclear receptor sub-family 1, group D, member 1 | Transcription factor | −1.23 | 6.34E-04 |
| Insulin secretion | |||||
| Metabolic processes | |||||
| Inflammatory processes | |||||
| MGI:2444521 | Rnf165 | Ring finger protein 165 | Zinc ion binding | −1.25 | 1.05E-02 |
| MGI:1351339 | Grm2 | Glutamate receptor, metabotropic 2 | Synaptic transmission | −1.27 | 2.12E-03 |
| Glutamate secretion | |||||
| MGI:102703 | Gng4 | Guanine nucleotide binding protein (G-protein), gamma 4 | Signal transduction | −1.42 | 7.64E-05 |
| GTPase activity | |||||
| Hemostasis | |||||
| Synaptic transmission | |||||
| Glucagon response | |||||
| Transmembrane transport of small molecules | |||||
| MGI:95295 | Egr1 | Early growth response 1 | Transcriptional regulator | −1.55 | 6.15E-07 |
| Immune response | |||||
| T cell differentiation |