Figure 3. Comparison of TF-chromatin dynamics by CLK and FRAP.
(A) Model fits of CLK data for Ace1-GFP binding to CUP1 in cells with two different expression levels of Ace1-GFP (low, blue curve; high, red curve; table S2). Inset: distributions of t1/2 values obtained from multiple independent fits of the Ace1-GFP or LacI-GFP CLK data (shown in (C); (7)). (B) FRAP of Ace1-GFP in cells with low Ace1-GFP levels. (C) Model fits of CLK data for LacI-GFP binding to the Lac array in cells with low (blue curve) or high (red curve) levels of LacI-GFP (table S2). (D) FRAP of LacI-GFP in cells with low LacI-GFP levels.