Figure 3.

Antiapoptotic and antioxidative effect of PCS extract in H₂O₂-treated INS-1 cells. INS-1 cells were treated without (−) or with various doses of PCS extract for 24 h, and 100 μM H₂O₂ was added for the last 1 h (a) or 30 min ((b)–(d)). (a) Protein levels of apoptotic signaling pathway were analyzed using western blotting. The representative blots (left) and the quantitative analysis of the band intensity by densitomery (right). The data are presented as a ratio of control. (b) ROS levels were measured. (c) Levels of anti-oxidative related proteins were analyzed using western blotting. (d), (e) GPX activity and SOD activity were measured. Resveratrol (Res) was used as a positive control. *P < 0.05 versus (−)/H₂O₂.