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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Apoptosis. 2014 May;19(5):883–894. doi: 10.1007/s10495-014-0971-6

Fig. 3.

Fig. 3

Pro-apoptotic effect of apigenin on human prostate cancer cells. a Effect of apigenin on the protein expression of cytochrome C, Bax, Bcl2, Bcl-xL and Bad in PC-3 cells. The cells were treated with 20 μM apigenin for specified times and Western blotting was performed. Time-dependent increase in cytochrome C, Bax and Bad and a simultaneous decrease in Bcl2 and Bcl-xL protein expression was observed after apigenin treatment. β-actin was used as loading control. Numeric values represent the protein level normalized to the loading control (β-actin). b Bax/Bcl2 ratio was increased in time-dependent manner after 20 μM apigenin treatment favoring apoptosis. Details are described in “Materials and methods” section