Table 2.
Identification by MALDI-MS and ESI-LC MS/MS of proteins secreted by T. reesei Rut C-30 grown in CMM and LCM
| Number | ProtID a | Protein name | Precursor mass (kDa) | pI b | Mascot score c | Peptides matched | Sequence coverage (%) |
|---|---|---|---|---|---|---|---|
| 1d |
123989 |
Cellobiohydrolase I (Cel7A) |
55.4 |
4.6 |
73 |
5 |
13 |
| 2d |
123989 |
Cellobiohydrolase I (Cel7A) |
55.4 |
4.5 |
73 |
5 |
13 |
| 3d |
123989 |
Cellobiohydrolase I (Cel7A) |
55.4 |
4.5 |
73 |
5 |
13 |
| 4d |
123989 |
Cellobiohydrolase I (Cel7A) |
55.4 |
4.5 |
73 |
5 |
13 |
| 5d |
123989 |
Cellobiohydrolase I (Cel7A) |
55.4 |
4.5 |
73 |
5 |
13 |
| 6e |
123989 |
Cellobiohydrolase I (Cel7A) |
54.1 |
4.4 |
598 |
21 |
24.9 |
| 7e |
123989 |
Cellobiohydrolase I (Cel7A) |
54.1 |
4.5 |
201 |
8 |
16.7 |
| 8d |
72567 |
Cellobiohydrolase II (Cel6A) |
50.3 |
4.8 |
107 |
10 |
27 |
| 9d |
72567 |
Cellobiohydrolase II (Cel6A) |
50.3 |
4.9 |
100 |
9 |
27 |
| 10d |
72567 |
Cellobiohydrolase II (Cel6A) |
50.3 |
5.0 |
83 |
8 |
26 |
| 11d |
72567 |
Cellobiohydrolase II (Cel6A) |
49.6 |
5.1 |
133 |
5 |
13.4 |
| 12e |
72567 |
Cellobiohydrolase II (Cel6A) |
35.1 |
4.7 |
471 |
22 |
21.4 |
| 13e |
72567 |
Cellobiohydrolase II (Cel6A) |
49.6 |
5.5 |
94 |
5 |
6.6 |
| 14e |
72567 |
Cellobiohydrolase II (Cel6A) |
39.2 |
4.6 |
135 |
8 |
9.2 |
| 15d |
120312 |
Endoglucanase II (Cel5A) |
48.7 |
5.8 |
56 |
4 |
24 |
| 16e |
120312 |
Endoglucanase II (Cel5A) |
44.1 |
4.5 |
340 |
23 |
30.4 |
| 17e |
120312 |
Endoglucanase II (Cel5A) |
44.1 |
4.7 |
219 |
5 |
18.4 |
| 18e |
122081 |
Endoglucanase I (Cel7B) |
48.2 |
4.6 |
106 |
4 |
9.2 |
| 19d |
123232 |
Endoglucanase III (Cel12A) |
25.1 |
4.5 |
83 |
8 |
32 |
| 20d |
76672 |
β-Glucosidase I (BGLI) |
78.7 |
6.4 |
196 |
20 |
31.2 |
| 21d |
76672 |
β-Glucosidase I (BGLI) |
78.7 |
6.5 |
106 |
17 |
23.6 |
| 22d |
123283 |
Arabinofuranosidase (ABFI) |
40.2 |
5.5 |
113 |
11 |
25.1 |
| 23d |
49081 |
Xyloglucanase (Cel74A) |
87.3 |
5.2 |
184 |
15 |
29 |
| 24d |
49081 |
Xyloglucanase (Cel74A) |
87.3 |
5.4 |
123 |
11 |
24.7 |
| 25d |
121127 |
β-Xylosidase (BXLI) |
87.5 |
5.5 |
188 |
21 |
41 |
| 26d |
121127 |
β-Xylosidase (BXLI) |
87.5 |
5.6 |
132 |
15 |
24.1 |
| 27d |
121127 |
β-Xylosidase (BXLI) |
87.5 |
5.7 |
101 |
14 |
26.3 |
| 28d |
121127 |
β-Xylosidase (BXLI) |
87.5 |
5.8 |
155 |
17 |
28.6 |
| 29e |
74223 |
Xylanase I (XYNI) |
24.6 |
4.7 |
142 |
3 |
9.4 |
| 30d |
111849 |
Xylanase IV (XYNIV) |
53.4 |
5.9 |
139 |
10 |
24 |
| 31e |
73638 |
Cellulose-binding protein (CIPI) |
32.9 |
4.8 |
317 |
13 |
20.3 |
| 32d |
123940 |
Cellulose-binding protein (CIPII) |
49.0 |
6.3 |
107 |
13 |
29 |
| 33d |
56996 |
Mannanase I (MANI) |
40.1 |
4.8 |
97 |
8 |
19.2 |
| 34d |
73643 |
Endoglucanase IV (Cel61A) |
40.2 |
4.9 |
86 |
2 |
6.1 |
| 35d | 121418 | Lipolytic enzyme (GDSL) | 39.3 | 5.7 | 113 | 11 | 34 |
aFrom The Genome Portal of the Department of Energy Joint Genome Institute (JGI) for T. reesei; bpI determined by 2-DE; canalyzed by dMALDI-MS or eESI-LC MS/MS. 2-DE, two-dimensional gel electrophoresis; CMM, cane molasses medium; ESI-LC MS/MS, electrospray ionization liquid chromatography tandem mass spectrometry; LCM, lactose-based conventional medium; MALDI-MS, matrix-assisted laser desorption/ionization mass spectrometry.