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. Author manuscript; available in PMC: 2014 Aug 27.
Published in final edited form as: Nature. 2013 Dec 11;506(7489):507–510. doi: 10.1038/nature12892

Extended Data Figure 9. EDA-DA labeling of C. trachomatis is apparent as early as eight hours post infection.

Extended Data Figure 9

L2 cells infected with C. trachomatis (a) 6, (b) 8, and (c) 10 hours post infection grown in the presence of 1 mM EDA-DA. Time points examined covered 4, 6, 8, 10, 12, 18, 24, and 40 hour infected cells. Subsequent binding of the probe to a modified Alexa Fluor 488 (green) was achieved via a click chemistry reaction. Antibody to chlamydial MOMP (red) was used to label chlamydial EBs and RBs. Experiments were conducted in technical duplicates, and the time course was conducted three independent times, with between 3–5 fields viewed per time point per technical replicate.