Table 1. Summary of Published LC–MSn-Based DNA Adductomics Studies.
| approach | instrument | sample type | adduct type | details | ref |
|---|---|---|---|---|---|
| CNL | triple quad | in vitro | PhIPa | early report of MS adductomics | (77) |
| triple quad | in vitro animal tissues | IQb | electrospray at ∼500 nL/min | (73) | |
| triple quad | in vitro | polyaromatic hydrocarbons | column switching 500–650 Da | (78) | |
| pseudo-CNL | triple quad | human lung | screening of unknowns | method development adductome map data analysis | (35) |
| triple quad | human lung and esophagus | screening of unknowns | seven adducts unambiguously detected | (74) | |
| triple quad | various human tissues | screening of unknowns | adducts detected at ∼1 adduct per 107 bases | (72) | |
| triple quad | food (Quorn, button mushrooms, brewer’s yeast) | screening of unknowns | 7 SRM transitions per injection | (79) | |
| triple quad | in vitro | micronucleus test-positive compounds | application to micronucleus test | (75) | |
| triple quad | human gastric mucosa | screening of unknowns | first report of lipid peroxidation-related adducts in human | (36) | |
| DD–MS2 | Q-TOF | in vitro cells | melphalan | column switching, 5 μl/min | (80) |
| DD–CNL–MS3 | ion trap | human hepatocytes (treated) | 4-ABPc | human samples examined MS3 data acquisition | (71) |
| rat liver | MeIQxd | ||||
| buccal cells (in vivo) | tobacco constituents | ||||
| ion trap | in vitro | illudin S | used similar method to ref (71) | (76) |
a
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP).
b
2-Amino-3-methylimidazo[4,5-f]quinolone (IQ).
c
4-Aminobiphenyl (4-ABP).
d
2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx).