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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2013 Oct 17;20(1):26–36. doi: 10.1016/j.bbmt.2013.10.009

Figure 1. Adoptively transferred HY-specific CD8 T-cells expand and produce GVHD in allogeneic males.

Figure 1

Lethally irradiated B6 male mice received 5×106 T-cell (CD3) depleted marrow on Day 0, followed by 1×107 HY-specific CD8 by tail vein injection on Day +7. Clinical GVHD scoring was performed once weekly. (A) Composite GVHD scores on Day +60 posttransplant were consistent with sublethal GVHD, particularly manifested as (B) changes in skin integrity, fur ruffling and posture/activity, compared with no clinical signs of GVHD in syngeneic female recipients of female BMT. GVHD scoring data represent 4 independent experiments with 6–10 mice/group. (C) HY-specific CD8 were enumerated from spleen and bone marrow of transplant recipients by flow cytometry 7 and 28 days after adoptive transfer (Day +14 and +35 post-BMT) by gating on live, CD8a+CD45.2+ cells. Representative flow plots (left panel) and total numbers of CD8a+CD45.2+ cells per 100,000 splenocytes (right panel, top) or bone marrow cells (left panel, bottom) are shown.