Table 1.
Sequences of the PCR primers and siRNA oligos
| Gene | Forward | Reverse |
|---|---|---|
| mPGC-1α | GCAGGTCGAACGAAACTGAC | CTCAGCCTGGGAACACGTTA |
| CTGCTCTGGTTGGTGAGGAa | GCAGGCTCATTGTTGTACTGa | |
| mPEPCK | GGTGTTTACTGGGAAGGCATC | CAATAATGGGGCACTGGCTG |
| mSHP | GTACCTGAAGGGCACGATCC | ACCAGGGCTCCAAGACTTCAC |
| mG6Pase | CATCAATCTCCTCTGGGTGG | TGCTGTAGTAGTCGGTGTCC |
| hPGC-1α | CCCAGATCTTCCTGAACTTG | GCTAGCAAGTTTGCCTCATTC |
| 18S | CGCCGCTAGAGGTGAAATTC | CCAGTCGGCATCGTTTATGG |
| mCycs | CCAAATCTCCACGGTCTGTTC | ATCAGGGTATCCTCTCCCCAG |
| mATP5b | GGTTCATCCTGCCAGAGACTA | AATCCCTCATCGAACTGGACG |
| mCox4i1 | ATTGGCAAGAGAGCCATTTCTAC | CACGCCGATCAGCGTAAGT |
| mCox1 | ATTCCTTCATGTCGGACGAG | ACTGAGAAGCCCCCTCAAAT |
| mCox2 | ACGAAATCAACAACCCCGTA | GGCAGAACGACTCGGTTATC |
| mNRF1 | AGCACGGAGTGACCCAAAC | TGTACGTGGCTACATGGACCT |
| mKLF15 | GAGACCTTCTCGTCACCGAAA | GCTGGAGACATCGCTGTCAT |
| mFOXO1 | CCCAGGCCGGAGTTTAACC | GTTGCTCATAAAGTCGGTGCT |
| mHNF-4α | GTGGCGAGTCCTTATGACACG | GCTGTTGGATGAATTGAGGTTGG |
| hPEPCK | Hs00159918_m1 (Life Technologies) | |
| mAMPKα1(Prkaa1) | Mm01296700_m1 (Life Technologies) | |
| mAMPKα2(Prkaa2) | Mm01264789_m1 (Life Technologies) | |
| siRNA SIRT1 | AAATGTCTCCACGAACAGC | GCTGTTCGTGGAGACATTT |
| siRNA neg control | AM4635, AM4637, AM4390844 (Life Technologies) | |
| esiRNA EGFP | EHUEGFP (Sigma-Aldrich) | |
| esiRNA mouse Prkaa1 | EMU190491 (Sigma-Aldrich) | |
| esiRNA mouse Prkaa2 | EMU052501 (Sigma-Aldrich) |
a
For adenoviral PGC-1α detection.