Figure 6.

Inhibition of 5-LOX product formation by RF-Id in human whole blood. (A) Human whole blood was pre-incubated with the test compounds (or DMSO as vehicle) for 15 min at 37°C, followed by stimulation with 30 μM A23187 for 10 min at 37°C. (B) Human whole blood was primed for 15 min at 37°C with 1 μg·mL⁻¹ LPS, treated with the test compounds (or DMSO as vehicle) for 15 min at 37°C, and then stimulated with 1 μM fMLP. After 15 min at 37°C, the formation of 5-LOX products was determined (right panel). *P <0.05, **P <0.01, ***P <0.001 versus vehicle control. Data are expressed as percentage of vehicle (0.1% DMSO); means + SEM; n = 3.